Journal: Scientific Reports
Article Title: Secondary structural choice of DNA and RNA associated with CGG/CCG trinucleotide repeat expansion rationalizes the RNA misprocessing in FXTAS
doi: 10.1038/s41598-021-87097-y
Figure Lengend Snippet: Circular dichroism spectra and EMSA corresponding to DNA and RNA CGG sequences. ( A – F ) CD spectra and ( G – I ) PAGE showing the preference for the following conformations by DG1, DG5, DG6, RG1, RG5, and RG6: ( A ) B-form duplex (0.05 M KCl)/intermediate conformation (3 M KCl) (DG1), ( B , C , G , H ) intermolecular quadruplex (DG5 and DG6), ( D ) A-form duplex (RG1) and ( E , F , I ) intramolecular quadruplex (RG5 and RG6). ( J ) Schematic diagram illustrating the arrangement of G- and C-quadrats (taken from PDB ID: 1EVO) in a parallel RNA and DNA CGG quadruplex. This figure was generated by using pymol 1.3 ( www.pymol.com ). The arrows indicate the increase or decrease in the ellipticity concomitant with the change in the secondary structure (see the text for more details). The figures ( A – F ) were plotted by using MATLAB 7.11.0 software ( www.mathworks.com ). The EMSA ( G – I ) samples were analysed by 14% native PAGE and stained with ethidium bromide (EtBr). The unprocessed gel images ( G – I ) are incorporated in Supplementary Fig. A–C.
Article Snippet: MATLAB 7.11.0 ( www.mathworks.com ) software was used for plotting the graphs.
Techniques: Circular Dichroism, Generated, Software, Clear Native PAGE, Staining